We have developed methods that allow detection, quantitation, purification, and
identification of cardiac proteinsS-thiolated during ischemia and reperfusion. Cysteine was
biotinylated and loaded into isolated rat hearts. During oxidative stress, biotin-cysteine forms
a disulfide bond with reactive protein cysteines, and these can be detected by probing
Western blots with streptavidin-horseradish peroxidase. S-Thiolated proteins were purified
using streptavidin-agarose. Thus, we demonstrated that reperfusion and diamide treatment …