Molecular cloning of cDNA and a chromosomal gene encoding GPE1‐BP, a nuclear protein which binds to granulocyte colony‐stimulating factor promoter element 1
M Nishizawa, N Wakabayashi-Ito, S Nagata - FEBS letters, 1991 - Wiley Online Library
M Nishizawa, N Wakabayashi-Ito, S Nagata
FEBS letters, 1991•Wiley Online LibraryGranulocyte colony‐stimulating factor (G‐CSF) is produced from macrophages in response
to lipopolysaccharide (LPS). GPE1, a cis‐acting element of the G‐CSF gene promoter,
functioned as an LPS‐inducible element. We isolated cDNA and a chromosomal gene
encoding the mouse GPEI‐binding protein (GPE1‐BP). A 150‐amino acid protein deduced
from the cDNA has a basic domain and a leucine zipper motif, and seems to be identical to
that of recently isolated Ig/EBP. The nuclear extract from COS cells transfected with the …
to lipopolysaccharide (LPS). GPE1, a cis‐acting element of the G‐CSF gene promoter,
functioned as an LPS‐inducible element. We isolated cDNA and a chromosomal gene
encoding the mouse GPEI‐binding protein (GPE1‐BP). A 150‐amino acid protein deduced
from the cDNA has a basic domain and a leucine zipper motif, and seems to be identical to
that of recently isolated Ig/EBP. The nuclear extract from COS cells transfected with the …
Granulocyte colony‐stimulating factor (G‐CSF) is produced from macrophages in response to lipopolysaccharide (LPS). GPE1, a cis‐acting element of the G‐CSF gene promoter, functioned as an LPS‐inducible element. We isolated cDNA and a chromosomal gene encoding the mouse GPEI‐binding protein (GPE1‐BP). A 150‐amino acid protein deduced from the cDNA has a basic domain and a leucine zipper motif, and seems to be identical to that of recently isolated Ig/EBP. The nuclear extract from COS cells transfected with the cDNA showed GPE1‐binding activity. Transcripts were ubiquitously detected, and may be spliced from two exons of a single gene.
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