Publisher Summary This chapter focuses on the development and use of protein chimeras
(derived from proteins naturally present in the medusa, aequorea victoria) that are
specifically targeted to the mitochondria, either to the matrix or to the intermembrane space,
and can be used to monitor calcium and pH. Aequorin, the pioneer of the targeted
recombinant probes, is a Ca 2+-sensitive photoprotein that emits light on binding of Ca 2+ to
its three high affinities Ca 2+-binding sites. The chapter describes the use of mutants of …