[HTML][HTML] Ex vivo culture rescues hematopoietic stem cells with long-term repopulating capacity following harvest from lethally irradiated mice
JP Chute, J Fung, G Muramoto, R Erwin - Experimental hematology, 2004 - Elsevier
OBJECTIVE: High-dose ionizing radiation can cause lethal myeloablation in exposed
individuals. We examined whether ex vivo culture could rescue hematopoietic stem cells
with repopulating capacity following harvest from lethally irradiated animals. METHODS: We
exposed B6. SJL mice to 1050 cGy, harvested their irradiated bone marrow (BM), and
examined whether ex vivo culture of the irradiated BM mononuclear cells (MNC) with
porcine microvascular endothelial cells (PMVEC) or cytokines alone could rescue …
individuals. We examined whether ex vivo culture could rescue hematopoietic stem cells
with repopulating capacity following harvest from lethally irradiated animals. METHODS: We
exposed B6. SJL mice to 1050 cGy, harvested their irradiated bone marrow (BM), and
examined whether ex vivo culture of the irradiated BM mononuclear cells (MNC) with
porcine microvascular endothelial cells (PMVEC) or cytokines alone could rescue …
