[HTML][HTML] Bim, a proapoptotic protein, up-regulated via transcription factor E2F1-dependent mechanism, functions as a prosurvival molecule in cancer
Proapoptotic Bcl-2 homology 3-only protein Bim plays an important role in Bax/Bak-
mediated cytochrome c release and apoptosis. Here, we provide evidence for a novel
prosurvival function of Bim in cancer cells. Bim was constitutively overexpressed in multiple
prostate and breast cancer cells as well as in primary tumor cells. Quantitative real time PCR
analysis showed that Bim was transcriptionally up-regulated. We have identified eight
endogenous E2F1-binding sites on the Bim promoter using in silico analysis. Luciferase …
mediated cytochrome c release and apoptosis. Here, we provide evidence for a novel
prosurvival function of Bim in cancer cells. Bim was constitutively overexpressed in multiple
prostate and breast cancer cells as well as in primary tumor cells. Quantitative real time PCR
analysis showed that Bim was transcriptionally up-regulated. We have identified eight
endogenous E2F1-binding sites on the Bim promoter using in silico analysis. Luciferase …