Ontogeny of hematopoietic stem cell development: reciprocal expression of CD33 and a novel molecule by maturing myeloid and erythroid progenitors
C Brashem-Stein, DA Flowers, FO Smith, SJ Staats… - 1993 - ashpublications.org
C Brashem-Stein, DA Flowers, FO Smith, SJ Staats, RG Andrews, ID Bernstein
1993•ashpublications.orgWe have identified a molecule expressed by human marrow granulocyte/monocyte colony-
forming cells (CFU-GM), erythroid colony-forming cells (CFU-E), and erythroid burst-forming
units (BFU-E), but not their precursors detectable in long-term bone marrow culture. This
antigen, detected by flow microfluorimetry using monoclonal antibody 7B9, is coexpressed
with CD33 on many CD34+ CFCs, although only the 7B9 antigen was detected on a portion
of BFU-E and CFU-E, whereas only CD33 was found on a portion of CFU-GM. Antibody 7B9 …
forming cells (CFU-GM), erythroid colony-forming cells (CFU-E), and erythroid burst-forming
units (BFU-E), but not their precursors detectable in long-term bone marrow culture. This
antigen, detected by flow microfluorimetry using monoclonal antibody 7B9, is coexpressed
with CD33 on many CD34+ CFCs, although only the 7B9 antigen was detected on a portion
of BFU-E and CFU-E, whereas only CD33 was found on a portion of CFU-GM. Antibody 7B9 …
Abstract
We have identified a molecule expressed by human marrow granulocyte/monocyte colony-forming cells (CFU-GM), erythroid colony- forming cells (CFU-E), and erythroid burst-forming units (BFU-E), but not their precursors detectable in long-term bone marrow culture. This antigen, detected by flow microfluorimetry using monoclonal antibody 7B9, is coexpressed with CD33 on many CD34+ CFCs, although only the 7B9 antigen was detected on a portion of BFU-E and CFU-E, whereas only CD33 was found on a portion of CFU-GM. Antibody 7B9 appears to be useful for isolating subsets of progenitors based on their common or selective expression of 7B9 antigen and CD33.
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