Activation of multiple JAKs, STATs, SOCS, and apoptosis-associated proteins in the liver after injection of Con A. (a) and (b) C57BL/6J mice were injected with Con A (10 μg/g) at various time points. Total liver protein extracts and RNA were prepared and analyzed by Western blotting and RT-PCR (indicated by asterisks), respectively, using Ab’s and primers as indicated. Data are representative of three independent experiments with similar results. p, phosphorylated form.